Autoclaving and gas sterilization are common effective methods. If aseptic technique and instruments cannot be used, a scientific justification must be provided in the protocol which will be reviewed by the IACUC and approved on a case-by-case basis.Īseptic technique includes preparation of the animal subject and the surgeon-including attire, scrub, and gloves sterilization of instruments, supplies, and implanted materials and the use of operative techniques to reduce the likelihood of infection. Please ensure that your animal use applications specify that all recovery surgeries will be done using aseptic technique. To aid you in this goal, written surgical guidelines for rodents are provided. Exceptions to these guidelines can sometimes be made but must be fully justified and approved in advance by the IACUC. The incorporation of aseptic technique into surgical practice can reduce the risk of nosocomial infections associated with surgery. Aseptic technique is used to prevent the contamination of surgical wounds and other sterile/susceptible sites by organisms. This requirement is met by utilizing the surgery room contained within the Boston University Animal Science Center (BU ASC) and/or, in the case of rodents, identifying a dedicated area within your laboratory for animal surgery. ![]() Sterile technique is a set of specific practices and procedures performed to make equipment and areas free from all microorganisms and to maintain that sterility (BC Centre for Disease Control, 2010). In addition, the regulations require a dedicated area for all surgeries performed on vertebrate animals. Surgical asepsis is the absence of all microorganisms within any type of invasive procedure. This means setting up and maintaining a sterile field and utilizing sterile technique throughout the surgery. To inform Canberra Health Services (CHS) staff on the correct implementation of Standard Aseptic Technique and Surgical Aseptic Technique requirements. Therefore, please be advised that ALL survival surgeries performed on any vertebrate animal (including rodents), whether considered major (open body cavity) or minor, must employ aseptic technique. ![]() surgical hand hygiene is required prior to major invasive procedures such as surgery or central. BU IACUC Revised January 2014, Revised May 2019Īs stated in the Guide for the Care and Use of Laboratory Animals, which our assurance with the federal government requires us to follow, both major as well as minor surgical procedures on laboratory animals require aseptic technique and instruments (cf. The type of hand hygiene will depend on the procedure e.g.
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